BACGro Fluid Thioglycollate Medium w/ L&T, Bottle / 200ml

BACGroTM Fluid Thioglycollate Medium with Lecithin and Polysorbate 80 is intended for laboratory use as a general non-selective growth media for a wide variety of organisms, when neutralization of disinfectants is necessary. It is not intended for use in diagnosis, treatment, or prevention of disease in humans.

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Fluid Thioglycollate Medium is a general-purpose medium that supports the growth of both aerobic bacteria as well as anaerobic bacteria without the use of an anaerobic chamber. The inclusion of reducing agents lowers the oxygen content of the broth. This allows obligate aerobes and obligate anaerobes to grow in the same sample- obligate aerobes will grow at the top of the medium while obligate anaerobes will be able to grow in the bottom of the tube. Facultative organisms may grow throughout the medium. Casein Peptone and Yeast Extract provide the nitrogen source, along with essential vitamins for growth. The oxidation-reduction potentials of the medium are lowered by sodium thioglycollate and L-cystine. Resazurin acts as the oxidation indicator and will turn pink in the presence of oxygen. Dextrose is included as a carbon source while sodium chloride maintains osmotic balance. Lecithin and Polysorbate 80 are added to neutralize germicidal agents and disinfectants, which reduces their inhibitory effect.

Kit Specifications
Formulation (per Liter)

Casein Peptone 15.0 g
Yeast Extract 5.0 g
Dextrose 5.5 g
Sodium Chloride 2.5 g
L-Cystine 0.5 g
Resazurin 0.001 g
Agar 0.75 g
Polysorbate 80 5.0 g
Lecithin 0.7 g
Sodium Thioglycollate 0.5 g
Total 35.45 g/L

2-25 °C
Bacillus spp., Clostridia, General Growth, Mesophilic & Thermophilic Spoilage, Pseudomonas spp., Staphylococci
Product Attachments
  • Instruction for Use - PLM3275 Rev 02 Fluid Thioglycollate Medium with L&P 80, Prepared