BACGroTM D/E Neutralizing Broth, when prepared as directed, is intended for use in neutralizing and testing antimicrobial agents and disinfectants. D/E Neutralizing Broth is not intended for use in diagnosis, treatment, or prevention of disease in humans.
BACGroTM D/E Neutralizing Broth does not contain Polysorbate 80 and must be added during preparation for procedures requiring D/E Neutralizing Broth containing Polysorbate.
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D/E Neutralizing Broth is commonly used for environmental sampling in areas where antimicrobial compounds may be used. The composition of D/E Neutralizing Broth effectively neutralizes a broad spectrum of antimicrobial compounds, allowing organisms to grow in the presence of otherwise bacteriostatic agents. The inclusion of lecithin serves to neutralize quaternary ammonium compounds. Sodium thiosulfate neutralizes iodine and chlorine. Sodium Thioglycollate neutralizes mercurial while sodium bisulfite neutralizes aldehydes. Polysorbate 80, if added, neutralizes phenolic compounds.
Casein peptone and yeast extract provide a nitrogen source and essential vitamins for growth. Dextrose serves as the main energy source. The inclusion of Bromocresol Purple allows for the detection of glucose utilization, as the resulting pH shift will cause the medium to turn from purple to yellow.
