BACGro NFL Aseptic Validation Medium / 50kg

BACGroTM National Food Laboratory (NFL) Aseptic Validation Medium is used for the growth and isolation of mesophiles and thermophiles from food and food processing facilities. Aseptic Validation Medium is not intended for use in diagnosis, treatment, or prevention of disease in humans.

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National Food Laboratory Aseptic Validation Medium is used as a general medium to promote growth of aerobic bacteria and facultative anaerobic bacteria present in spoiled food products and is suitable for use in the validation and commissioning of aseptic filling processes. It is designed to recover spore forming organisms that are used to validate many common sterilization processes, such as steam, hydrogen peroxide, or peracetic acid.

Casein peptone provides a source of carbon and nitrogen required for growth of organisms while yeast extract serves as a source of essential vitamins and nutrients. The presence of glucose serves as a fermentable carbohydrate for organisms such as Geobacillus stearothermophilus and other flat sour organisms. The production of acid from glucose is detected by the inclusion of bromocresol purple, which turns from purple to yellow in response to acid production.

Kit Specifications
Formulation (per Liter)

Casein Peptone 10.0 g
Dextrose 5.0 g
Yeast Extract 1.0 g
Bromocresol Purple 0.04 g
Total 16 g/L

2-30 °C
Bacillus spp., Mesophilic & Thermophilic Spoilage
Product Attachments
  • Instructions for Use - National Food Laboratory Aseptic Validation Medium