BACGroTM Fluid Thioglycollate Medium, when prepared as directed, is intended for use in cultivating aerobic and anaerobic non-fastidious bacteria for sterility testing. Fluid Thioglycollate Medium is not intended for use in diagnosis, treatment, or prevention of disease in humans.
BACGroTM Fluid Thiglycollate Medium conforms to the harmonized USP/EP/JP and the FDA Bacteriological Analytical Manual.
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Fluid Thioglycollate Medium is a general-purpose medium that supports the growth of both aerobic bacteria as well as anaerobic bacteria without the use of an anaerobic chamber. The inclusion of reducing agents lowers the oxygen content of the broth. The medium also contains a small amount of agar that results in an oxygen gradient from the top of the tube to the bottom. This allows obligate aerobes and obligate anaerobes to grow in the same sample- obligate aerobes will grow at the top of the medium while obligate anaerobes will be able to grow in the bottom of the tube. Facultative organisms may grow throughout the medium.
Casein Peptone and Yeast Extract provide the nitrogen source, along with essential vitamins for growth. The oxidation-reduction potentials of the medium are lowered by Sodium Thioglycollate and L-Cystine. Resazurin acts as the oxidation indicator and will turn pink in the presence of oxygen. Dextrose is included as a carbon source while sodium chloride maintains osmotic balance. The addition of agar limits the diffusion of oxygen throughout media.
