Okadaic Acid (DSP), PP2A, Plate, 96 tests

This protocol specifies a method for the quantitative determination of Okadaic Acid (OA) and other carboxylic toxins of the OA group including DTX1, DTX2 and DTX3 by a colorimetric phosphatase inhibition assay. This method is applicable to shellfish species such as mussels, clams, oysters and scallops. Test based on the phophatase activity inhibition by OA-toxins group, responsible for diarrheic shellfish poisoning (DSP). Phosphatase enzyme PP2A is able to hydrolyse a specific substrate, yielding a product that can be detected at 405 nm. Samples containing toxins from the okadaic acid group will inhibit the enzyme activity proportionally to the amount of toxin contained in the sample. The concentration of toxin in the sample can be calculated using a standard curve.