Immunoassay for the quantitative and sensitive screening of Monensin.
The Monensin ELISA is an immunoassay for the quantitative and sensitive screening of Monensin. This test is suitable for the quantitative and/or qualitative screening of Monensin in animal feed and contaminated products. For additional matrices, contact Eurofins Abraxis technical services for application bulletins and/or specific matrix validation guidelines. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of Monensin by specific antibodies. Monensin, when present in a sample, and a Monensin-HRP analogue compete for the binding sites of sheep anti-Monensin antibodies in solution. The Monensin antibodies are then bound by a second antibody (donkey anti-sheep) immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Monensin present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.