Kit for a duplex GMO quantitation of CaMV 35s promoter and AgroBorder II soy (48 reactions) with soy DNA (le1) reference PCR (48 reactions)
Details
Kit for a duplex GMO quantitation of CaMV 35s promoter and AgroBorder II soy (48 reactions) with soy DNA (le1) reference PCR (48 reactions)
The screening methods CaMV 35S promoter and AgroBorder II element are an ideal combination for GMO testing of soybean samples. GMOQuant Duplex 35S/AgroBorder II Soy provides an increased lab efficiency by combining the CaMV 35S promoter and AgroBorder II quantification for soybean in one analytical step. The Kit provides materials for the simultaneous detection and the quantitative measurement of the 35S promoter, a regulatory DNA element derived from the Cauliflower Mosaic Virus (CaMV), and the AgroBorder II modification in DNA extracted from soybean samples or products with soy content.
Kit Specifications
SKU
5125222301
No. of tests
96
Max no. of samples
9
Specificity
CaMV 35S promoter and AgroBorder II element quantification in soybean
Relevant species
Soybean
Detection limit (LOD)
LODasym = 1/10.000;
LOD95% ≤ 10 copies / target;
LODrel ≤ 0.01%
Time to Result
~2.5 h (~90 min PCR duration)
PCR cycler platforms
Applied Biosystems 7500 or the Agilent Mx3000PTM/ Mx3005P.
Kit components
- BasicMix 35S / AgroBorder II: GS-P-26.009 EFGi2x QNTP (NR) 2U, composed of PCR reaction mix, dNTPs, hot-start polymerase, buffer; 1 tube for 48 reactions each (625 µL), # -01: 1 x 625 µL, # -10: 10 x 625 µL,# -50: 50 x 625 µL
- OligoMix 35S / AgroBorder II: OligoMix QN RT IPC (LR/HR+) GS-P-07.108 35S/ABII, composed of primers, probe, dNTPs, 1 tube for 48 reactions each (375 µL); # -01: 1 x 375 µL, # -01: 1 x 375 µL, # -10: 10 x 375 µL, # -50: 50 x 375 µL
- BasicMix / Soy Lectin: GS-P-26.008 EFG 2x QN TP (NR) 1U, composed of PCR reaction mix, dNTPs, hot-start polymerase, buffer; 1 tube for 48 reactions each (625 µL); # -01: 1 x 625 µL, # -10: 10 x 625 µL, # -50: 50 x 625 µL
- OligoMix / Soy Lectin: OligoMix QN RT(LR/HR+), GS-P-25.010 Soybean Lectin1, composed of primers, probe, dNTPs, 1 tubes for 48 reactions each (375 µL); # -01: 1 x 375 µL, # -10: 10 x 375 µL, # -50: 50 x 375 µL
- Calibration Standards 1-5 for soy reference / lectin1 and GM soy (35S/AgroBorder II): # -01: 5 x 65 µL, # -10: 10 x 5 x 65 µL, # -50: 25 x 5 x 65 µL