GENESpin is designed for the isolation of genomic DNA from food and feed samples of plant and animal origin.
It allows processing of up to 200 mg material (larger amounts are possible through upscaled use of lysis buffer). Typical yields for GENESpin are in the range of 0.1-10 µg DNA. The eluted DNA is ready for use in subsequent reactions like PCR or real-time PCR.
Details
GENESpin is designed for the isolation of genomic DNA from food and feed samples of plant and animal origin.
It allows processing of up to 200 mg material (larger amounts are possible through upscaled use of lysis buffer). Typical yields for GENESpin are in the range of 0.1-10 µg DNA. The eluted DNA is ready for use in subsequent reactions like PCR or real-time PCR.
Kit Specifications
SKU
5224400605
No. of tests
50 samples
Sample material
at least 200 mg
Fragment size
300 bp - approx. 50 kbp
Binding capacity
30 µg
Elution volume
100 µL
Preparation time
30 min/6 preps
Specificity
• Kit for 50 DNA extractions
• Recommended sample types: food and feed samples • Silica-based columns • Fast: DNA extraction in less than two hours • Comfortable handling • For extraction from 200 mg sample • Yields very pure DNA suitable for PCR and real-time PCR applications • High DNA yield: typically 0.1 – 10 μgKit components
• GENESpin Lysis Buffer
• GENESpin Binding Buffer • GENESpin Wash Buffer 1 and 2 • GENESpin Elution Buffer • GENESpin Proteinase Buffer • GENESpin Proteinase K • GENESpin Columns • Collection tubesEquipment needed
• Water bath or incubator for 65°C and 70°C
• Heating block for 65°C • Pipettors • Microcentrifuge • Vortex mixer • Equipment for sample disruption/preparation/homogenization