Cry1F lateral flow strips (LFS) test kit for bulk grain corn is intended to be used for the qualitative detection of Cry1F protein present in corn. The kit detects one Cry1F corn seed in 200 conventional corn seeds (0.5%)
The total incubation time of the assay is 10 minutes.
Details
An antibody specific to the Cry1F protein molecule is immobilized on the test line area of nitrocellulose membrane. Second antibody specific for Cry1F molecule is conjugated with colloidal gold and incorporated in the sample area of lateral flow strip. Anti mouse IgG is immobilized on the control line area of the LFS.
When the LFS is placed in the sample extract, the Cry1F protein present in the sample extracts binds to the antibody labelled with gold nano particles and the complex moves upward by capillary action. The complex then binds to the antibody coated on the test line resulting in pink/purple color test line. As the complex moves further up, it binds to the control line resulting in pink/purple color control line. In absence of Cry1F, the test line does not appear as no complex binds to the test line while control line turns pink/purple color indicating validity of test protocol.
Kit Specifications
SKU
AID 050
No. of tests
100
Max no. of samples
100
Specificity
Qualitative detection of Cry1F protein present in corn. The kit detects one Cry1F corn seed in 200 conventional corn seeds (0.5%).
Relevant species
Bulk grain corn
Cross-reactivities
The Cry1F test kit does not cross react with CP4EPSPS, Cry1Ac, Cry1Ab, Cry2A, Vip3A, Cry34Ab, Cry35Ab1, Cry3B, mCry3A, eCry3.1Ab and PAT ( Liberty Link***).
Assay time
10 min
Storage
2 - 8° C
Kit components
Cry1F LFS strips 50 strips per canister: Two canisters per kit; One pack insert