BACGroTM Violet Red Bile Agar w. X-GLN (VRBX), when prepared as directed, is intended for the detection and enumeration of Escherichia coli and other coliforms from food and dairy products.
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Violet Red Bile Agar with X-GLN contains peptone to provide vitamins and minerals, as well as a carbon and nitrogen source. Yeast extract promotes bacterial growth by supplying B-complex vitamins. Lactose provides a carbohydrate source. Bile salts and crystal violet aid in the inhibition of Gram-positive bacteria. Neutral red is a pH indicator in which lactose-fermenters will produce red colonies and halos (due to bile precipitation). Sodium chloride conserves osmotic balance. X-glucuronide is a chromogenic substrate for β-glucuronidase. Agar is a solidifying agent.
The presence of β-glucuronidase differentiates most E. coli from other coliforms. The formed E. coli colonies are colored blue.
Peptone 7.0 g
Yeast Extract 3.0 g
Lactose 10.0 g
Bile Salts No. 3 1.5 g
Sodium Chloride 5.0 g
Neutral Red 0.03 g
Crystal Violet 0.002 g
X-Glucuronide 0.1 g
Agar 15.0 g
Total 41.6 g/L
