ELISA test kit for the quantitative detection of corticosteroids.
Details
ELISA test kit for the quantitative detection of corticosteroids.
Advantages: B ZERO® is the most cost-effective line of ELISA test kits. Thanks to the removal of the calibrators, the analysts are not required to use wells for the standard curve. Quantitative results are obtained by means of a virtual master-curve that is provided within each kit batch.
Kit Specifications
SKU
HU0050008
Assay principle
Microplate competitive enzyme immunoassay without standard curve.
Format
Microtiter plate with 96 wells
Assay time
75 min
Analysable samples
Feed, Urine, Milk, Liver, Tissue
Detection limit (LOD)
- Urine - 0.25 ppb
- Liver, Tissue - 0.20 ppb
- Milk - 0.05 ppb
- Feed - 5.00 ppb
Sample preparation
- Urine - Centrifugation or filtration, dilution
- Liver, Tissue - Homogenization, Extraction in Acetonitrile, Centrifugation, Evaporation
- Milk - Centrifugation, Extraction in Acetonitrile, Centrifugation, Evaporation
- Feed - Extraction in Axcetonitrile, Dilution
Specificity
- Dexamethasone 100%
- Flumethasone 70%
- Betamethasone <0.1%
- Triamcinolone <0.1%
- Prednisolone 20%
- Cortisol 2%
- Cortisone <1%
- Methylprednisolone <1%