Magnetic particle kit for detection of Permethrin and related pyrethroids.
For detection of Permethrin and related pyrethroids (please refer to cross-reactivity table) in water (groundwater, surface water, well water). Please refer to the attached specific procedures for water (groundwater, surface water, well water). For soil, crop, and food use contact Eurofins Abraxis for application bulletins and/or specific matrix validation guidelines. The Pyrethroid Assay applies the principles of enzyme-linked immunosorbent assay (ELISA) to the determination of Permethrin and related pyrethroids. The sample to be tested is added, along with paramagnetic particles attached with antibodies specific to pyrethroids, to a disposable glass test tube, and incubated for 20 minutes. This is followed by the addition of a pyrethroid enzyme conjugate. Both the pyrethroids (which may be in the sample) and the enzyme-labeled Permethrin analog (the enzyme conjugate) compete for antibody binding sites on the magnetic particles. At the end of a thirty minute (30) incubation period, a magnetic field is applied to hold the paramagnetic particles (with Pyrethroid and labeled Permethrin analog bound to the antibodies on the particles, in proportion to their original concentration) in the tube and allow the unbound reagents to be decanted. After decanting, the particles are washed with Washing Solution. The presence of pyrethroids is detected by adding the Color Solution, which contains enzyme substrate (hydrogen peroxide) and the chromogen (3,3',5,5'-tetramethyl-benzidine). The enzyme-labeled Permethrin analog bound to the Pyrethroid antibody catalyzes the conversion of the substrate/chromogen mixture to a colored product. After an incubation period of thirty (30) minutes, the reaction is stopped and stabilized by the addition of acid. Since the labeled Permethrin (conjugate) was in competition with the unlabeled Pyrethroids (sample) for the antibody sites, the color developed is inversely proportional to the concentration of Pyrethroids in the sample.