The Saxitoxin ELISA is an immunoassay for the quantitative and sensitive detection of Saxitoxin in water and shellfish samples. Saxitoxin is one of the toxins associated with paralytic shellfish poisoning (PSP).
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The Saxitoxin ELISA is an immunoassay for the quantitative and sensitive detection of Saxitoxin in water samples. Saxitoxin is one of the toxins associated with paralytic shellfish poisoning (PSP). For shellfish samples, sample preparation is required. The test is a direct competitive ELISA based on the recognition of Saxitoxin by specific antibodies. Saxitoxin, when present in a sample, and a saxitoxin-enzyme conjugate compete for the binding sites of rabbit antisaxitoxin antibodies in solution. The saxitoxin antibodies are then bound by a second antibody (anti-rabbit) immobilized on the microtiter plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of the Saxitoxin present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA plate reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
