ELISA test kit for the detection of sulphonamides.
Details
ELISA test kit for the detection of sulphonamides.
Advantages: B ZERO® is the most cost-effective line of ELISA test kits. Thanks to the removal of the calibrators, the analysts are not required to use wells for the standard curve. Quantitative results are obtained by means of a virtual master-curve that is provided within each kit batch.
Kit Specifications
SKU
HU0050006
Assay principle
Microplate competitive enzyme immunoassay.
Format
Microtiter plate with 48 wells
Assay time
75 min
Analysable samples
Muscle, Egg, Milk, Honey, Feed
Detection limit (LOD)
- Muscle, Egg (Method I), Honey (Method III, high sensitivity) - 0.5 ppb
- Milk (Method I) - 0.25 ppb
- Muscle, Egg, Milk, Honey (Method II, solvent-free) - 5 ppb
- Feed - 66.6 ppb
Sample preparation
- Muscle, Egg, milk (METHOD I, high sensitivity): Homogenization, (skimming for milk), solvent extraction, centrifugation, evaporation.
- Muscle, egg, honey (METHOD II, solvent free): Homogenization, (acid hydrolysis for honey), buffer extraction, centrifugation, filtration.
- Milk (Method II) - Centrifugation, Dilution
- Honey (Method III) - Acidic Hydrolysis, Purification on SPE Column, Elution, Evaporation
- New line for Feed - Grinding, Solvent Extraction, Dilution
Specificity
- Sulfamerazine >100%
- Sulfamonomethoxine >100%
- Sulfadiazine >100%
- Sulfaclozine (Sulfachloropyrazine) > 100%
- Sulfachloropyridazine >100%
- Sulfamethoxypyridazine (Sulfapyridazine) >100%
- Sulfamethoxydiazine (Sulfamethoxine, Sulfameter) >100%
- Sulfadimethoxine >100%
- Sulfaquinoxaline >100%
- Sulfathiazole >100%
- Sulfamethizole >100%
- Sulfamethazine 100%
- Sulfamethoxazole 90%
- Sulfisoxazole 50%
- Sulfapyridine 36%
- Sulfadoxine 24%
- Sulfacetamide 2.5%
- Sulfaphenazole <2%
- Sulfabenzamide <2%
- Sulfaguanidine <0.1%
- Sulfanilamide <0.1%