Test kit for the qualitative detection of bovine and porcine DNA with 2 x 48 real-time PCR reactions
Details
Test kit for the qualitative detection of bovine and porcine DNA with 2 x 48 real-time PCR reactions
Kit Specifications
SKU
5422212010
No. of tests
2 x 48
Time schedule
DNA-extraction 2 h
PCR-reaction 4 h
Total time 6 h
Kit components
- (1x) OligoMix: OM QL RT IPC (LR/HR+)Module GS-P-08.030 Cattle
- 390 µL primer/probe mix for bovine DNA detection
- (1x) OligoMix: OM QL RT IPC (LR/HR+) Module GS-P-08.041 Pig
- 390 µL primer/probe mix for bovine DNA detection
- (2x) BasicMix: BM (NR) GS-P-26.012 EFGi TP 2x (NR) 1.5U
- 650 µL reaction mix without primers and probes
- (2x) ROX™ dye, 23 µL
- (1x) Genomic DNA Cattle [Bos taurus] 50 µL
- (1x) Genomic DNA Pig [Sus scrofa domestica]50 µL
- (2x) DNA stabilization buffer (for NTCs)
Detection limit (LOD)
LODabs ≤ 10 copies; LODrel ≤ 0.01%
PCR cycler platforms
Agilent MX3005P/3000P, Agilent AriaMX, ABI7500/Fast, Bio-Rad CFX Touch,Roche LC480 and ABI 7900HT
Material & Equipment not included
- water, DNase-free
- Vortex
- Micropipettes (variable 1-1000 µL) and filter tips
- PCR optical tubes or plates 0.2 ml and optical caps or seals
- RT-PCR Thermocycler
Storage
-20°C