Test kit for the qualitative real-time PCR detection of bovine DNA (detects besides Bos taurus also bison and several other Bos species).
Details
Test kit for the qualitative real-time PCR detection of bovine DNA (detects besides Bos taurus also bison and several other Bos species).
Kit Specifications
SKU
5422220610
No. of tests
96
Time schedule
DNA-extraction 2 h
PCR-reaction 4 h
Total time 6 h
Kit components
- 2x MasterMix QL RT IPC (LR/HR+) GS-P-08.030, Cattle
- 650 µL BasicMix QL RT IPC (NR)
- 390 µL OligoMix QL RT IPC (LR/HR+)
- 2 x Positive control DNA: Genomic DNA from Cattle
- 1x DNA stabilization buffer, 150 µL (for NTCs)
- 2x ROX™ dye, 23 µL (to be used only for High Rox cyclers)
Detection limit (LOD)
The relative limit of detection has been validated at 0.01% of bovine DNA in other species (total DNA content of 200ng/rxn)
PCR cycler platforms
Agilent MX3005P/3000P/Aria MX, ABI7500/Fast, Roche LC480, ABI 7900HT and BioRad CFXTouch96
Material & Equipment not included
- water, DNase-free
- Vortex
- Micropipettes (variable 1-1000 µL) and filter tips
- PCR optical tubes or plates 0.2 ml and optical caps or seals
- RT-PCR Thermocycler
Storage
-20°C