Immunoassay for the quantitative and sensitive detection of Histamine.
The Histamine Rapid ELISA is an immunoassay for the quantitative and sensitive detection of Histamine in fish and wine. If necessary, positive samples can be confirmed by HPLC or other conventional methods. The test is a direct competitive ELISA that allows the detection of Histamine. It is based on the recognition of Histamine by specific antibodies. Histamine, when present in a sample, and a Histamine-AP analog compete for the binding sites of rabbit anti-Histamine antibodies that have been immobilized onto the microtiter wells. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the yellow color is inversely proportional to the concentration of the Histamine present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the calibration curve constructed with each run.
Sandwich enzyme immunoassay
Microtiter plate with 96 wells
17 min incl. derivatization
0; 2, 6; 12; 24; 72 ppm
450 nm (HRP + TMB)
Detection limit (LOD)
N-Acetyhistamine 0.02% 1-Methylhistamine 5% Histidine 0% Serotonin 0%