eCry3.1Ab ELISA kit

eCry3.1Ab ELISA test kit is intended to be used for the qualitative detection of eCry3.1Ab chimeric protein present in transgenic Maize plant carrying Syngenta event 5307 (Agrisure Duracade©). The test is used to detect the eCry3.1Ab protein in individual Maize seed or leaf. It can also be used to detect the Syngenta event 5307 in bulk grain at 0.2 % (1 positive in 500 negative seeds).

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Different quantities are also available in 10/50 plate formats. 
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Details
An antibody specific to the eCry3.1Ab protein molecule is immobilized on the microwell plates and the second antibody specific for eCry3.1Ab protein is conjugated with horse radish peroxidase ( HRP). When the sample extracts are added to the microtiter wells, the eCry3.1Ab from sample binds to the antibody immobilized in the well. This binding is subsequently detected by addition of enzyme-labeled antibody. After a washing step, the substrate is added. The enzymatic reaction and development of color is proportional to the amount of eCry3.1Ab present in the sample. The reaction is terminated by the addition of stop solution. Absorbance is then measured on a plate reader.
Kit Specifications
SKU
AID 051
No. of tests
96
Specificity
Qualitative detection of eCry3.1Ab chimeric protein present in transgenic Maize plant carrying Syngenta event 5307 (Agrisure Duracade©)
Relevant species
Corn leaf, seed and bulk grain
Cross-reactivities
The eCry3.1Ab ELISA kit does not cross-react with CP4EPSPS (Roundup Ready**), Cry1F, Cry1Ac, mCry3A (AgriSure RW), Cry3Bb, Cry1Ab, Cry2A, Vip3A, Cry34Ab1, Cry35Ab1, Cry1A.105 or PAT (LibertyLink***)
Assay time
60 min for leaf/seed & 90 min for bulk grain
Storage
2-8 °C