Cry1Ab LFS kit for Corn bulk grain ( 0.8 %)

Cry1Ab lateral flow strips (LFS) test kit for bulk grain corn is intended to be used for
the qualitative detection of Cry1Ab protein present in Cry1Ab corn. The kit detects one
Cry1Ab corn in 125 conventional corn seeds ( 0.8 %)
The total incubation time of the assay is 10 minutes.

Details

An antibody specific to the Cry1Ab protein molecule is immobilized on the test line area of nitrocellulose
membrane. Second antibody specific for Cry1Ab molecule is conjugated with colloidal gold and incorporated in
the sample area of lateral flow strip. Anti mouse IgG is immobilized on the control line area of the LFS.


When the LFS is placed in the sample extract, the Cry1Ab protein present in the sample extracts binds to the
antibody labelled with gold nano particles and the complex moves upward by capillary action. The complex then
binds to the antibody coated on the test line resulting in pink/purple color test line. As the complex moves further
up, it binds to the control line resulting in pink/purple color control line. In absence of Cry1Ab the test line does
not appear as no complex binds to the test line while control line turns pink/purple color indicating validity of test
protocol.

Kit Specifications
SKU
AID 045
No. of tests
100
Max no. of samples
100
Specificity
Qualitative detection of Cry1Ab protein present in Cry1Ab corn. The kit detects one Cry1Ab corn in 125 conventional corn seeds ( 0.8 %)
Relevant species
Bulk grain corn
Cross-reactivities
The Cry1Ab test kit does not cross react with CP4EPSPS, Cry1F, Cry2A, Vip3A, Cry34Ab, Cry35Ab1, Cry3B, mCry3A, eCry3.1Ab and PAT ( Liberty Link***).
Assay time
10 min
Storage
2 - 8° C
Kit components
Cry3Bb LFS strips 50 strips per canister:Two canisters per kit; One pack insert