Transplant Diagnostics

Transplant Diagnostics

CMV Tetramer Test

Progressive CMV infections pose a major threat to transplant recipients. CD8 T cells are considered to be the primary control for CMV viremia. Sufficient levels of CMV-specific CD8 T cells are found to protect against CMV disease (see references 1,2). Current assays based on PCR and antigen-detection are capable of analysing the viral burden and detect a CMV-reactivation, however, any immunoreactivtity against CMV cannot be assessed.

The CMV-Tetramer Test provides for the first time a ready-to-use kit to quantify the number of CMV-specific CD8 T cells, thus helping the clinician to assess patients with high risk to develop CMV disease.

Assay Features:

  • Standardized and CE-certified ready-to-use staining kit (performance time about 2h).
  • Direct analysis of whole bloodMonitoring CMV-specific T cells.
  • Highly specific and sensitive detection of CMV-specific CD8 T cells with tetramers.
  • Combination of well characterized immunodominant CMV peptides and HLA-types (A1, A2, B7).
  • Determination of the absolute numbers of CMV-specific CD8 T cells and the percentage CD4 and CD8 T cells.
  • Kit contains all necessary compounds including a negative control.
  • Easy and economic procedure based on flow cytometry achieving quick results at low costs with high accuracy and precision.

Expected benefits of monitoring the CMV-specific CD8 T cell number (see references 1-5):

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1) Gratama & Cornelissen, Clin. Appl. Immun.Rev. 2001 
2) Moss & Khan, Hum. Immun. 2004 
3) Cwynarski et al. Blood 2001 
4) Gratama et al. Blood 2001 
5) Gratama et al., Cytometry B Clin. Cytom. 2008


Immune Cell Function

Glycotope Biotechnology offers several clinical diagnostics and research reagents based on flow cytometry procedures for the fast and precise measurement of various cell functions focused on functional characterisation of the cellular immune reponse and characterisation of haematological relevant auto-immune disease.

Our Core Competencies:

We offer a range of flow cytometry-based assays for the analysis of the functional activity of

  • Neutrophilic granulocytes
  • Monocytes
  • Basophilic granulocytes
  • Natural killer cells

Clinical diagnostic for the quantitative determination of the chemotactic function of neutrophilic granulocytes
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Clinical diagnostic for the quantitative analysis of leukocyte phagocytosis in human whole blood
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Clinical diagnostic for the quantitative analysis of leukocyte oxidative burst in human whole blood
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Clinical diagnostic for the quantification of the cytotoxic activity of human natural killer cells
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Clinical diagnostic for the determination of the allergen-induced basophil degranulation
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Research Reagent for the quantification of platelets-associated immunoglobulin
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Research Reagent for the determination of reticulated platelets and low platelets count
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*According to the EU’s in-Vitro Diagnostic (IVD) Directive